CD16/CD32, clone: 93, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0161-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis. 93 has also been reported in blocking of Fc-mediated reactions in functional studies. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with 0.5-1 μg of purified anti-CD16/CD32 per million cells for 5-10 minutes on ice prior to staining. (Please use Functional Grade purified 93, cat. 16-0161, in functional assays.).Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|FCGR3, FCGR3A, FCGR3B, CD16A, CD16B, Fc gamma Receptor III, Fc gamma Receptor 3, RP11-5K23.1, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, FCGR2, CD32, Fc gamma Receptor II, CD32 receptor 2, Fc gamma Receptor 2, FCGR2A, FCGR2B, FCGR2C, Low affinity immunoglobulin gamma Fc region receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, FCG2, Low affinity immunoglobulin gamma Fc region receptor II-c, Fc-gamma RII-c, Fc-gamma-RIIc, FcRII-c, FGFR2B|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.