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CD157, PE-Cyanine7, clone: eBioSY11B5 (SY11B5), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-1579-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15548496

  • £75.29 / 25 tests


Estimated Shipment date
from Supplier 28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1). CD157 is a 42-45 kDa, GPI-anchored protein with structural and functional similarities with CD38. CD157 was initially cloned because of its expression on monocytes and macrophages, and was subsequently discovered to be the same protein named BST-1, discovered for its expression on bone marrow stromal cells and its ability to stimulate the proliferation of a mouse pre-B cell line. CD157 is a pleiotropic ectoenzyme and is thought to act independently as an enzyme and receptor. Similar to CD38, CD157 is involved in the metabolism of NAD+ and this activity may be involved in regulating intracellular Ca2+ levels. As a receptor, upon binding of its putative ligand, CD157 is thought to initiate a signal transduction cascade resulting in the phosphorylation of cytoplasmic proteins including focal adhesion kinase (FAK). The mechanism and functional significance of CD157-initiated signal transduction remain to be fully characterized.Applications Reported: This eBioSY11B5 (SY11B5) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioSY11B5 (SY11B5) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD157
eBioSY11B5 (SY11B5)
PE-Cyanine7
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
BST-1, BP-3/IF-7
IgG1
RUO
Primary
Human
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