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PeproTech CD154 (CD40 Ligand) (soluble) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Product Code. 17854123
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2 x 500 μg
25 μg
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500 μg
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1mg
25µg
500µg
50µg
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Product Code. Quantity unitSize
17854123 25 μg 25µg
17894793 50 μg 50µg
17804383 2 x 500 μg 1mg
30054452 500 μg 500µg
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Product Code. 17854123 Supplier PeproTech Supplier No. 500P142GBT25UG

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Description

Goat Polyclonal Antibody

AA Sequence of recombinant protein: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human sCD40 Ligand. Anti-Human sCD40 Ligand-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human sCD40 Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sCD40 Ligand (500-P142G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sCD40 Ligand. Western Blot: To detect hsCD40L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsCD40L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P142GBT-1MG will be provided as 2 x 500 μg

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD154 (CD40 Ligand) (soluble)
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene CD40LG
Gene Accession No. P29965
Gene Alias CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5
Gene Symbols CD40LG
Host Species Goat
Immunogen E.coli-derived Recombinant Human sCD40 Ligand.
Purification Method Antigen affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 959
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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