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CD152 (CTLA-4), Functional Grade, clone: 9H10, eBioscience™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-1521-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288567

  • £100.02 / 100µg


Estimated Shipment
29-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 9H10 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.Applications Reported: The 9H10 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro functional studies.Applications Tested: The 9H10 antibody has been tested by flow cytometric analysis of resting and Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD152 (CTLA-4)
9H10
Unconjugated
Purified
Golden Syrian Hamster
100μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
CTLA4
IgG
RUO
Primary
Murine
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