CD146 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA528893

Product Code. 13403017

Description

Recommended positive controls: HUVEC, rat liver extract. Predicted reactivity: Mouse (81%), Rat (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Specifications

• Antigen: CD146
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MCAM
• Gene Alias: 1-gicerin; AV025631; CD146; CD149; Cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; l-gicerin; l-gicerin precursor; l-gicerin protein; LOW QUALITY PROTEIN: cell surface glycoprotein MUC18; MCAM; melanoma adhesion molecule; melanoma cell adhesion molecule; Melanoma-associated antigen A32; melanoma-associated antigen MUC18; Muc18; mucin-18; s-endo; S-endo 1 endothelial-associated antigen; s-gicerin; s-gicerin precursor
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4162, 78967, 84004
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.21 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P43121, Q8R2Y2, Q9EPF2
• Gene Symbols: MCAM
• Immunogen: Recombinant fragment corresponding to a region within amino acids 421 and 646 of Human CD146
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG