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CD144 (VE-cadherin), clone: eBioBV13 (BV13), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1441-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15267227

  • £48.31 / 50µg


EU Stock 1
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Description

Description

Description: The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Intravenous injection of BV13 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs. (Please use Functional Grade purified eBioBV13 (BV13), cat. 16-1441, in functional assays.).Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD144 (VE-Cadherin)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P55284
Cadherin-5, Vascular endothelial cadherin, VE-cadherin, 7B4 antigen, CD144 antigen, CDH5
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)þImmunoprecipitationþWestern Blot
eBioBV13 (BV13)
Unconjugated
Cdh5
Purified
Cdh5
IgG1, kappa
50μg
Store at 2-8°C.
Primary
12562
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