CD137 (4-1BB) Monoclonal Antibody (17B5), eFluor™ 450, eBioscience™, Invitrogen™

Syrian Hamster Monoclonal Antibody

Product Code	Brand	Quantity
15589716	Affymetrix eBioscience 48-1371-82	100 μg
15579716	Affymetrix eBioscience 48-1371-80	25 μg

Description

Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays. Applications Reported: This 17B5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 17B5 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

Specifications

• Antigen: CD137 (4-1BB)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P20334
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 17B5
• Applications: Flow Cytometry
• Conjugate: eFluor 450
• Host Species: Syrian Hamster
• Target Species: Mouse
• Gene ID (Entrez): 21942
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.