CD135 Rat anti-Mouse, PE, Clone: A2F10.1, BD

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15860739	BD Biosciences 561068	25 μg
15847418	BD Biosciences 553842	0.1 mg

Description

The A2F10 monoclonal antibody specifically binds to Flk-2/Flt3 (Ly-72, CD135), a receptor protein tyrosine kinase closely related to c-kit, c-fms, and PDGF Receptor of the immunoglobulin superfamily. The Flt3 message is detected in hematopoietic stem cells and primitive progenitor cells in fetal liver, adult bone marrow, and fetal and adult thymus, as well as brain, placenta, and testis; but it is absent in more mature hematopoietic cells. In flow cytometric analysis, the A2F10 antibody recognizes Flt3-transfected Y3 cells (rat myeloma), but not the parent cell line in addition to recognizing early B lymphoid lineage cells in juvenile and adult bone marrow. A role for CD135 in the regulation of hematopoiesis is suggested by the observations that soluble Flk-2/Flt3 ligand can both stimulate proliferation of stem cell-enriched fetal liver, fetal thymus, and adult bone marrow populations and enhance their responses to other growth factors in vitro. In addition, injection of Flk-2/Flt3 ligand stimulates extramedullary hematopoiesis in the mouse spleen and accumulation of dendritic cells in the hematopoietic system. mAb A2F10.1 is reported to immunoprecipitate a 150-kDa surface protein from the murine myeloblast cell line M1, which naturally expresses CD135, and to inhibit the binding of Flk-2/Flt3 ligand to CD135.

Specifications

• Antigen: CD135
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Target Species: Mouse
• Isotype: IgG2a κ
• Purification Method: Affinity Purified
• Description: Flt3; Fms-like tyrosine kinase 3; FLT-3; FLK-2; Fetal liver kinase 2; Ly72

• Clone: A2F10.1
• Applications: Flow Cytometry
• Conjugate: PE
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.