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CD11c, PE-Cyanine5.5, clone: N418, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 35-0114-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15371020

  • £182.82 / 100µg


EU Stock 5
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Description

Description

Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90kDa heterodimer.Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis.Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-567 nm; Emission: 690 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Specifications

Specifications

CD11c
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9QXH4
AI449405, Cd11c, Cr4, N418, CD11 antigen-like family member C, ITGAX, integrin aX, integrin alpha-X
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
N418
PE-Cyanine5.5
Itgax
Conjugated
Itgax
IgG
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
16411
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