CD11b Rat anti-Mouse, PE-Cy7, Clone: M1/70, BD

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15850769	BD Biosciences 561098	25 μg
15826878	BD Biosciences 552850	0.1 mg

Description

The M1/70 antibody reacts with the 170kDa α[M] chain of Mac-1 (CD11b/CD18, α[M]β[2] integrin), also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1 (CD54). Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 cells. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. M1/70 antibody reportedly blocks cell adherence and C3bi binding, but it does not block cell-mediated lysis. Cross-reaction of mAb M1/70 with CD11b on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Rat
Clone: M1/70
Isotype: IgG2b κ
Species Reactivity: Mouse
Immunogen: Mouse splenic Cells

Flow Cytometry

Specifications

• Antigen: CD11b
• Concentration: 0.2mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Target Species: Human, Mouse
• Isotype: IgG2b κ
• Purification Method: Affinity Purified
• Description: Itgam; Integrin alpha-M; Ly-40; Mac-1a; Mac-1 alpha; CR3A; CR-3 alpha chain

• Clone: M1/70
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.