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CD11b/c, PerCP-eFluor™ 710, clone: OX42, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0110-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341230

  • £77.14 / 25µg


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Description

Description

Description: This OX42 monoclonal antibody reacts with rat CD11b and CD11c. CD11b, also known as integrin alpha M or Mac-1, is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, is a component of complement receptor 4 (CR4). CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.The OX42 antibody has been reported to inhibit complement-mediated rosette formation by granulocytes and macrophages. Moreover, studies demonstrate that OX42 can inhibit granulocyte aggregation.Applications Reported: This OX42 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX42 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD11b/c
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Conjugated
Itgam, Itgax
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
25021, 499271
Flow Cytometry
OX42
PerCP-eFluor 710
Itgax
Itgax, Itgam, CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, CD11bc
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
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