CD11b/c Monoclonal Antibody (OX-42), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA181606

Product Code. 11560023

Description

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Rat CD11b, clone OX-42, recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. Mouse anti Rat CD11b, clone OX-42, recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats.

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue. CD11c encodes the integrin alpha X chain protein. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.

Specifications

• Antigen: CD11b/c
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: 0
• Gene Symbols: Itgam, Itgax
• Immunogen: Rat peritoneal macrophages.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: OX-42
• Conjugate: Unconjugated
• Gene: Itgam
• Gene Alias: AI449405; antigen CD11b (p170); CD11 antigen-like family member B; CD11 antigen-like family member C; CD11b; CD11B (p170); CD11b/CD18; CD11bc; CD11C; CD11C (p150) alpha polypeptide; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component 3 receptor 4 subunit; complement component receptor 3 alpha-a; complement receptor 4; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; Cr4; F730045J24Rik; integrin alpha M; integrin alpha X; integrin alpha-M; Integrin alpha-X; integrin aX; integrin subunit alpha M; integrin subunit alpha X; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); integrin, alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAM; ITGAX; Leu M5; leu M5, alpha subunit; leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor MO1; leuk
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 25021, 499271
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid