CD11a Monoclonal Antibody (121/7), Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen MA11A5

Product Code. 11814301

Description

MA11A5 targets CD11a in FACS, IA, and Neu applications and shows reactivity with mouse samples. This antibody does not react with human Jurkat cells in FACS applications. The MA11A5 immunogen is mouse 180kD alpha chain (CD11alpha) of the integrin Lymphocyte Function-Associated Antigen-1. MA11A5 detects CD11a which has a predicted molecular weight of approximately 126 kDa. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified. This product is a Low Endotoxin formulation. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

Specifications

• Antigen: CD11a
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P24063
• Gene Symbols: ITGAL
• Immunogen: Mouse 180kD alpha chain (CD11alpha) of the integrin Lymphocyte Function-Associated Antigen-1
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Inhibition Assays, Neutralization
• Clone: 121/7
• Conjugate: Unconjugated
• Gene: ITGAL
• Gene Alias: (p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 16408
• Content And Storage: -20°C
• Form: Liquid