CD119 Mouse anti-Human, Brilliant Violet 510, Clone: GIR-208, BD Optibuild™

Mouse Monoclonal Antibody

Brand:  BD Biosciences 743027

Product Code. 15817959

Description

The GIR-208 antibody recognizes the extracellular region of CD119 which is also known as the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γreceptor (IFN-γRα). The functionally active-form of the human IFN-γreceptor consists of two (or more) subunits, with IFN-γRαresponsible for IFN-γbinding and both the IFN-γRαandβchains required for the transduction of biologic responses. The IFN-γreceptorαchain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. Binding of 125I-labeled GIR-208 antibody to IFN-γRα+ cells is reported to be specifically inhibited in the presence of excess IFN-γ. GIR-208 does not cross react with IFN-γas tested by ELISA. The ability of this antibody to bind to IFN-γreceptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γ

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: CD119
• Clone: GIR-208
• Conjugate: Brilliant Violet 510
• Gene Alias: IFN- gamma receptor alpha chain
• Immunogen: Human IFN-γRα
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ