CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), Super Bright™ 436, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 62-1159-41

Product Code. 15865503

Description

Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts. Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 12-3A3-1B10 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid maligcy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.

Specifications

• Antigen: CD115 (c-fms)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P07333
• Gene Symbols: CSF1R
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1436
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 12-3A3-1B10
• Conjugate: Super Bright 436
• Gene: CSF1R
• Gene Alias: C-FMS; CD115; CSF-1R; CSF1R; CSFR; FIM2; FMS; HDLS; M-CSF-R
• Host Species: Rat
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ