Invitrogen™ CD105 (Endoglin) Monoclonal Antibody (SN6), eFluor™ 450, eBioscience™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15539706	Invitrogen™ 48-1057-42	100 Tests

Description

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis. Applications Reported: This SN6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Specifications

• Antigen: CD105 (Endoglin)
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: eFluor 450
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P17813
• Gene ID (Entrez): 2022
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: SN6
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: ENG
• Gene Alias: AI528660; AI662476; CD105; CD105 antigen; cell surface MJ7/18 antigen; Edg; END; Endo; Endoglin; endoglin (Osler-Rendu-Weber syndrome 1); Eng; HHT1; ORW; ORW1; S-endoglin; transmembrane glycoprotein
• Gene Symbols: ENG
• Product Type: Antibody