Invitrogen™ Mouse CD154 (CD40 Ligand), Recombinant Protein

Recombinant Protein

Brand:  Invitrogen™ RP8641

Product Code. 11519161

Description

CD40L (mouse) Recombinant Protein, RP-8641, from Invitrogen.

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2. Recombinant mouse NGF-beta is a non-glycosylated, non-covalently linked homodimer, containing 149 amino acid chains , with a molecular weight of 16.4 kDa each. Purity is typically greater than 98% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the proliferation of TF-1 cells and is typically 3.7-5.5 ng/mL. The specific activity of this protein is 1x10^6 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL.

Specifications

• Accession Number: P27548
• For Use With (Application): Control
• Gene ID (Entrez): 21947
• pH Range: 7.5
• Source: E. Coli
• Regulatory Status: RUO
• Common Name: Mouse CD154 (CD40 Ligand)
• Specific Reactivity: Mouse
• Cross Reactivity: Ms
• Recombinant: Recombinant
• Protein Subtype: Recombinant

• Concentration: 0.1mg/mL
• Formulation: 10mM sodium phosphate with 50mM arginine and no preservative; pH 7.5
• Name: Mouse CD154 (CD40 Ligand)
• Quantity: 25μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Gene Alias: M-CD-40-L
• Gene Symbol: Cd40lg
• Conjugate: Unconjugated
• Species: E. coli
• Form: Lyophilized
• Purity or Quality Grade: purified