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Gibco™ CD FortiCHO™ Medium
Description
CD FortiCHO™ medium is fortified to support maximal recombinant CHO cell growth and protein expression in suspension batch culture, offering 120% greater growth and production than select competitive media with simple glucose supplementation. CD FortiCHO™ medium can provide up to 75% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with CD EfficientFeed™ C nutrient supplement.
When to Use CD FortiCHO™ Medium
When to Consider Another Gibco™ Medium
Consider CD OptiCHO™ medium when:
Consider CD CHO medium when:
Consider CD DG44 when:
Dry Media Formulations
For early access to CD FortiCHO™ AGT™ medium, please contact Gibco™ Technical Services.
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Specifications
Specifications
| Cell Line | CHO |
| Classification | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | CD OptiCHO AGT Medium |
| With Additives | Sodium Pyruvate, Sodium Bicarbonate |
| Without Additives | No Glutamine, No Phenol Red |
| Product Line | Gibco |
| Quantity | 1 L |
| Shipping Condition | Room Temperature |
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Frequently Asked Questions (FAQs)
Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.
SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.
Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.
For Research Use Only. Not for use in diagnostic procedures.
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