Invitrogen™ CCRL2 Chimeric Recombinant Rabbit Monoclonal Antibody (BZ2E3)

Rabbit Recombinant Monoclonal Antibody

Brand:  Invitrogen™ MA548143

Product Code. 17920761

Description

Specificity: This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding. Preparation: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

CCRL2 (C-C chemokine receptor-like 2), also known as chemokine receptor X, human chemokine receptor (HCR), CRAM-A or CRAM-B, is a seven pass transmembrane protein expressed by monocytes, neutrophils, and dendritic cells of immunal tissues (predominanly spleen, fetal liver, bone marrow and lymph node). It shares over 40% homology with other C-C chemokine receptors but does not share the conserved DRY motif which is among the important motifs necessary for signalling and ligand-binding. C-C chemokine receptors are G-protein coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation.

Specifications

• Antigen: CCRL2 Chimeric
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: O35457
• Gene Symbols: CCRL2
• Immunogen: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide (aa 1-24) /KLH conjugate.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG κ

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: BZ2E3
• Conjugate: Unconjugated
• Gene: CCRL2
• Gene Alias: 1810047I05Rik; Ackr5; atypical chemokine receptor 5; C-C chemokine receptor-like 2; C-C motif chemokine receptor like 2; CCR11; CCR6; Ccrl2; chemokine (C-C motif) receptor-like 2; chemokine C-C motif receptor-like 2; chemokine C-C motif receptor-like 2-like; chemokine receptor CCR11; Chemokine receptor X; CKRX; Cmkbr1l2; CRAM; CRAM-A; CRAM-B; E01; G-protein coupled beta chemokine receptor; HCR; Lccr; L-CCR; Lipopolysaccharide-inducible C-C chemokine receptor; LOC100719164; MGC116710; Putative MCP-1 chemokine receptor
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 54199
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid