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CCL8 (MCP-2), eFluor 660, clone: DWZEE, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9789-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15590137

  • £176.50 / 100 tests


Estimated Shipment date
from Supplier 28-06-2018
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Description

Description

Description: This DWZEE monoclonal antibody reacts with human CCL8. CCL8, also known as MCP-2 (Monocyte Chemotactic Protein 2), is a member of the CC- subfamily of chemokines and is most closely related to CCL2 (MCP-1) and CCL7 (MCP-3). They are secreted by a variety of cell types in response to inflammatory stimuli and play critical roles in the recruitment of leukocytes to areas of inflammation. While all three MCP proteins are potent chemoattractants of monocytes and T cells, CCL7 appears to have the broadest range of activity, as it has also been demonstrated to attract activated NK cells, eosinophils, basophils, and neutrophils. CCL8 has also been demonstrated to attract NK cells, eosinophils, and basophils, but requires higher concentrations to do so. CCL8 signals via the g protein-coupled receptor CCR5, which is shared with other CC-chemokines. CCR5 is the primary co-receptor for HIV entry, which the virus binds through the gp120 envelope protein. All CCR5 ligands demonstrate potent inhibition of virus entry into the cell, both through steric hindrance of gp120-CCR5 interaction, and ligand-mediated receptor internalization.Applications Reported: This DWZEE antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This DWZEE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood monocytes. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CCL8 (MCP-2)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P80075
HC14, MCP-2, MCP2, SCYA10, SCYA8
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryĆ¾Flow Cytometry
DWZEE
eFluor 660
CCL8
Conjugated
CCL8
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
6355
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