PeproTech CCL3 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17803313	PeproTech 500-P77BT-25UG	25 μg
17833023	PeproTech 500-P77BT-50UG	50 μg
17853103	Invitrogen 500-P77BT-1MG	2 x 500μg
30054603	PeproTech 500-P77BT-500UG	500 μg

Descrizione

AA Sequence of recombinant protein: APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MIP1alpha (CCL3). Anti-Rat MIP-1alpha (CCL3) specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Rat MIP-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat MIP-1alpha (500-P77) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MIP-1alpha. Western Blot: To detect Rat MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P77BT-1MG will be provided as 2 x 500 μg

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

Specifica

• Antigen: CCL3
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Rat
• Gene Accession No.: P50229
• Gene ID (Entrez): 25542
• Immunogen: E.coli-derived Recombinant Rat MIP-1alpha (CCL3).
• Purification Method: Antigen affinity chromatography
• Gene: Ccl3

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: AI323804; C-C motif chemokine 3; C-C motif chemokine ligand 3; CCL3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; G0S19-1; Heparin-binding chemotaxis protein; H-MIP-1-alpha; L2G25B; LD78ALPHA; LD78-alpha; LD78-alpha(4-69); Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP1 (a); mip1 alpha; MIP-1 alpha; MIP1-(a); MIP1A; MIP-1a; MIP-1alpha; MIP-1-alpha; MIP1-alpha; MIP-1-alpha(4-69); PAT 464.1; RP23-320E6.7; Scya3; SIS-alpha; SIS-beta; small inducible cytokine A3; small inducible cytokine A3 (homologous to mouse Mip-1a); small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein; TY-5
• Gene Symbols: Ccl3
• Primary or Secondary: Primary
• Content And Storage: -20°C