PeproTech CCL28 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P297BT-25UG

Product Code. 17825473

Description

AA Sequence of recombinant protein: SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MEC (CCL28). Anti-Human MEC (CCL28)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hMEC by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MEC (500-P297) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hMEC. Western Blot: To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P297BT-1MG will be provided as 2 x 500 μg

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor, and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.

Specifications

• Antigen: CCL28
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: CCL28
• Gene Alias: CC chemokine CCL28; C-C motif chemokine 28; C-C motif chemokine ligand 28; CCK 1; CCK1; CCK1 protein; CCL 28; Ccl28; chemokine (C-C motif) ligand 28; chemokine (C-C motif) ligand 28 splice variant chi; MEC; Mucosae-associated epithelial chemokine; Protein CCK1; SCYA28; small inducible cytokine A28; small inducible cytokine subfamily A (Cys-Cys), member 28; small-inducible cytokine A28
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56477
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q9NRJ3
• Gene Symbols: CCL28
• Immunogen: E.coli-derived Recombinant Human MEC (CCL28).
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody