CCL21 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen 500-P114BT-1MG

Product Code. 17834353

Description

AA Sequence of recombinant protein: SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine Exodus-2 (CCL21). Anti-Murine Exodus-2 (CCL21)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Murine Exodus-2 (CCL21) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Exodus-2 (CCL21) (500-P114) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Exodus-2 (CCL21). Western Blot: To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P114BT-1MG will be provided as 2 x 500 μg

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

Specifications

• Antigen: CCL21
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: Ccl21b
• Gene Alias: 6 Ckine; 6CKBAC1; 6CKBAC2; 6Ckine; AI159701; ALP; AW987545; beta chemokine exodus-2; beta-chemokine exodus-2; CC chemokine 6Ckine-ser; C-C motif chemokine 21; C-C motif chemokine 21a; C-C motif chemokine 21b; C-C motif chemokine 21c; C-C motif chemokine ligand 21; CCL21; Ccl21a; Ccl21b; Ccl21c; Ccl21-leu; CCL21-Ser; chemokine (C-C motif) ligand 21; chemokine (C-C motif) ligand 21A (serine); chemokine (C-C motif) ligand 21B (leucine); chemokine (C-C motif) ligand 21b (serine); chemokine (C-C motif) ligand 21C (leucine); chemokine CCL21/6CKINE; CKb9; ECL; Efficient Chemoattractant for Lymphocytes; exodus-2; plt; Scya21; SCYA21a; Scya21b; Scya21c; secondary lymphoid organ chemokine; secondary lymphoid tissue chemokine; Secondary lymphoid-tissue chemokine; SLC; small inducible cytokine A21; small inducible cytokine A21a; small inducible cytokine A21c; small inducible cytokine subfamily A (Cys-Cys), member 21; small-inducible cytokine A21; Small-inducible cytokine A21a; Small-inducible cyto
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100042493, 18829, 65956
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P84444, P86792, P86793
• Gene Symbols: Ccl21a, Ccl21b, Ccl21c
• Immunogen: E.coli-derived Recombinant Murine Exodus-2 (CCL21)
• Quantity: 2 x 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Form: Lyophilized