PeproTech CCL14 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  PeproTech 500-P106BT-25UG

Product Code. 17824143

Description

AA Sequence of recombinant protein: TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HCC-1 (CCL14). Anti-Human HCC-1 (CCL14)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human HCC-1 (CCL14) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human HCC-1 (CCL14) (500-P106) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human HCC-1 (CCL14). Western Blot: To detect hHCC-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hHCC-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P106BT-1MG will be provided as 2 x 500 μg

This gene, CCL14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts; it is unknown if its monocistronic transcript is also subject to alternative splicing.

Specifications

• Antigen: CCL14
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: CCL14
• Gene Alias: C-C motif chemokine 14; C-C motif chemokine ligand 14; CC-1; CC-3; CCL14; chemokine (C-C motif) ligand 14; chemokine CC-1/CC-3; chemokine CC-3; CKB1; H HCC-1(1-74); HCC-1; HCC-1(1-74); HCC-1(3-74); HCC-1(4-74); HCC-1(9-74); HCC-1/HCC-3; HCC-3; hemofiltrate CC chemokine 1; MCIF; NCC2; NCC-2; new CC chemokine 2; SCYA14; SCYL2; small inducible cytokine subfamily A (Cys-Cys), member 14; small-inducible cytokine A14; SY14
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6358
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q16627
• Gene Symbols: CCL14
• Immunogen: E.coli-derived Recombinant Human HCC-1 (CCL14) (72 a.a.).
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody