Invitrogen™ Caspase 9 (Cleaved Asp315) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5114689

Product Code. 17247453

Description

Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp315.

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer's Disease (AD).

Specifications

• Antigen: Caspase 9 (Cleaved Asp315)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CASP9
• Gene Alias: APAF3; APAF-3; apoptosis-related cysteine protease; apoptotic protease activating factor 3; apoptotic protease MCH-6; Apoptotic protease-activating factor 3; cas9; CASP9; CASP-9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; OTTHUMP00000002324; PPP1R56; protein phosphatase 1, regulatory subunit 56
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 842
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide
• Gene Accession No.: P55211
• Gene Symbols: CASP9
• Immunogen: A synthesized peptide derived from human CASP9(Accession P55211), corresponding to amino acid residues F296-A316.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG