Caspase 11, clone: 17D9, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9935-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody 17D9 recognizes mouse Caspase-11, a cysteine protease that plays a role in apoptosis and inflammation. Caspase-11 exists as a 43kDa and 38kDa pro-caspase form and a 30 and 10kDa active form. The 17D9 antibody recognizes p43, p38 and p30 forms. Caspase-11 is an upstream protease that activates other caspases, such as caspase-3, -7 and -1, which in turn activate IL-1beta. Under Caspase-11-deficient conditions, cells are unable to process or secrete IL-1beta in response to stimuli and show defects in motility, which are due to disruption of its interaction with Aip1, an actin binding protein. Caspase-11 is homologous to human Caspase-4 (around 60%). Unlike other caspases which tend to be constitutively expressed, caspase-11 is upregulated by LPS treatment in macrophages, lymphocytes and hepatocytes.Applications Reported: This 17D9 antibody has been reported for use in immunoblotting (WB).Applications Tested: This 17D9 antibody has been tested by western blotting at 1-5ug/ml on J774 cells or stimulated splenocyte lysates. Samples must be reduced for detection. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|ICE(rel)II, ICEREL-II, ICH-2, Mih1/TX, TX|
|Store at 2-8°C.|
For Research Use Only.