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Cas9 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA5143501

Additional Details : Weight : 0.01000kg

Product Code. 17836767

  • £1356.00 / 500µL
Estimated Shipment: 19-08-2024
to see stock.

Description

Description

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
Specifications

Specifications

Cas9
Polyclonal
Unconjugated
cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus
CAS9 from S. aureus expressed in and purified from E. coli
RUO
Bacteria
Antibody
IgG
Immunocytochemistry, Immunohistochemistry, Western Blot
Conc. Not Determined
whole serum with no preservative
Rabbit
500 μL
Primary
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Liquid
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