PeproTech Cardiotrophin 1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17864443	PeproTech 500-P101BT-25UG	25 μg
17804063	PeproTech 500-P101BT-50UG	50 μg
17884353	Invitrogen 500-P101BT-1MG	2 x 500 μg
30054745	PeproTech 500-P101BT-500UG	500 μg

Description

AA Sequence of recombinant protein: SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Cardiotrophin-1. Anti-Human Cardiotrophin-1-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human Cardiotrophin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Cardiotrophin-1 (500-P101) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Cardiotrophin-1. Western Blot: To detect hCardiotrophin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hCardiotrophin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P101BT-1MG will be provided as 2 x 500 μg

Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and has been shown to induce hypertrophy in cardiac myocytes in vitro. Cardiotrophin-1, a secreted protein expressed at high levels in myocardium during cardiogenesis, has been shown to promote proliferation and survival of embryonic cardiomyocytes, suggesting a role for Cardiotrophin-1 in the activation of gp130 during cardiac development.

Specifications

• Antigen: Cardiotrophin 1
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q16619
• Gene ID (Entrez): 1489
• Immunogen: E.coli-derived Recombinant Human Cardiotrophin-1.
• Purification Method: Antigen affinity chromatography
• Gene: CTF1

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: cardiophin 1; Cardiotrophin; cardiotrophin 1; Cardiotrophin1; cardiotrophin-1; CT 1; CT1; CT-1; CT-1CT1; CTF 1; Ctf1; IL-6 cytokine family
• Gene Symbols: CTF1
• Primary or Secondary: Primary
• Content And Storage: -20°C