Thermo Scientific Chemicals CAPS, 0.5M buffer soln., pH 10.5

Product Code. 15484819

Brand: Thermo Scientific Chemicals J62446.AK

Description

CAS: 1135-40-6 | C9H19NO3S | 221.32 g/mol

CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI>8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

Solubility
Soluble in water (100 g/L) at 20°C, and methanol (1%).

Notes
Store at room temperature. Ensure good ventilation. Incompatible with oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 1135-40-6
• Molecular Formula: C9H19NO3S
• Molecular Weight (g/mol): 221.32
• MDL Number: MFCD00003837
• InChI Key: PJWWRFATQTVXHA-UHFFFAOYSA-N
• IUPAC Name: 3-(cyclohexylamino)propane-1-sulfonic acid
• SMILES: OS(=O)(=O)CCCNC1CCCCC1

• Concentration: 0.5 M
• pH: 10.5
• Physical Form: Liquid
• Quantity: 250 mL
• Chemical Name or Material: CAPS

Safety and Handling

Product Identifier

• CAPS, 0.5M buffer solution, pH 10.5

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : 3-(cyclohexylamino)-1-propanesulfonic acid

SDS

RUO – Research Use Only