Calprotectin Monoclonal Antibody (27E10), Biotin, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA140314

Product Code. 10009733

Description

MA1-40314 detects Calprotectin from human and non-human primate samples. Does not cross react with mouse. MA1-40314 has been successfully used in ELISA, immunohistochemistry (frozen), flow cytometry, immunofluorescence, immunoprecipitation and Western blot applications. For Western blot, detects the non-reduced protein at about 22 kDa. The MA1-40314 immunogen is proprietary.

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

Specifications

• Antigen: Calprotectin
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.1% BSA and 0.02% sodium azide
• Gene Accession No.: P05109, P06702
• Gene Symbols: S100A8, S100A9
• Immunogen: Human blood monocytes
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human, Non-human Primate, Rhesus Monkey
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Clone: 27E10
• Conjugate: Biotin
• Gene: S100A9
• Gene Alias: 60B8Ag; AI323541; AW546964; B8Ag; BEE11; BEE22; CAGA; CAGB; calcium binding protein; S100 protein; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin; calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; EGK_01322; EGK_21438; GAGB; intracellular calcium-binding protein (MRP14); L1AG; Leukocyte L1 complex heavy chain; leukocyte L1 complex light chain; LIAG; LOC100735514; LOC532569 protein; MA387; MAC387; macrophage migration inhibitory factor-related protein-14; macrophage migration inhibitory factor-related protein-8; MIF; migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; Mrp14; MRP-14; MRP8; MRP-8; myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; neutrophil cytosolic 7 kDa protein; NIF; p14; p23; P7; p8; Pro-inflammatory S100 cytokine; protein S100-A8; Protein S100-A9; S100 calcium binding protein A8;
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 6279, 6280, 714690, 714740
• Content And Storage: 4° C
• Form: Liquid