Invitrogen™ Calpain S1/S2 Monoclonal Antibody (28F3)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA112433

Product Code. 11588113

Description

This antibody specifically recognizes the 28 to 30 kD small subunit of Calpain 1 and Calpain 2 (mu calpain and m calpain). It recognizes both native and denatured protein in ELISA, Western Blot and Immunoprecipitation.

The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and m-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, m-calpain is active at micromolar (mM) concentrations of Ca++. In addition to the ubiquitously expressed m- and m- calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Specifications

• Antigen: Calpain S1/S2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 3mM sodium azide; pH 7.5
• Gene Accession No.: P04632, P13135, Q96L46
• Gene Symbols: CAPNS1, CAPNS2
• Immunogen: Human Calpain protein purified from placenta.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Bovine
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunoprecipitation, Western Blot
• Clone: 28F3
• Conjugate: Unconjugated
• Gene: CAPNS1
• Gene Alias: 2310005G05Rik; 30K-2; calcium-activated neutral proteinase 2; Calcium-activated neutral proteinase small subunit; Calcium-dependent protease small subunit; Calcium-dependent protease small subunit 1; calcium-dependent protease small subunit 2; calcium-dependent protease, small subunit; calpain 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain 4; calpain 4, small subunit (30K); calpain II regulatory subunit; calpain II regulatory subunit (EC 3.4.22.17); calpain large polypeptide L2; Calpain M-type; calpain regulatory subunit; Calpain small subunit 1; calpain small subunit 2; calpain, large polypeptide L2; calpain, small polypeptide; calpain, small subunit 1; calpain, small subunit 2; calpain-2 catalytic subunit; Calpain-2 large subunit; CALPAIN4; CANP; CANP 2; CANP small subunit; CANP2; CANPL2; CANPml; CANPS; Capa4; Capa-4; CAPN2; Capn4; CAPNS; CAPNS1; Capns2; CDPS; CSS1; CSS2; D7Ertd146e; M-calpain; mCANP; Millimolar-calpain
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 100300424, 281664, 826, 84290
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid