Invitrogen™ Calpain 6 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA589430

Product Code. 16567331

Description

Immunogen sequence: MGPPLKLFKN QKYQELKQEC IKDSRLFCDP TFLPENDSLF YNRLLPGKVV WKRPQDICDD PHLIVGNISN HQLTQGRLGH KPMVSAFSCL AVQESHWTKT IPNHKEQEWD PQKTEKYAGI FHFRFWHFGE WTEVVIDDLL PTINGDLVFS FSTSMNEFWN ALLEKAYAKL LGCYEALDGL TITDIIVDFT GTLAETVDMQ KGRYTELVEE KYKLFGELYK TFTKGGLICC SIESPNQEEQ EVETDWGLLK GHTYTMTDIR KIRLGERLVE; Positive Samples: Rat heart; Cellular Location: Cytoplasm, cytoskeleton, perinuclear region, spindle.

Calpain-6, also known as calpamodulin or calpain-X, is an intracellular, calcium-dependent cysteine protease. Calpain-6 has a much more tissue-specific expression in adults than the ubiquitous calpain-1 and calpain-2, and has thus far been found primarily in the placenta, although it is expressed embryonically in a number of tissues. The classical calpain family members consist of a common small subunit (calpain-4), and a large variable subunit, but it is not known if calpain-6 associates with a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain T in calpains 5 and 6). Located on the X chromosome, the calpain-6 sequence lacks the EF hand calcium-binding motif found in domain-IV of the classical calpains. In addition, the canonical active site Cys, His and Asn are modified to Lys, His, Asn in human and Lys, Tyr, Asn in mouse calpain-6, making it unlikely that calpain-6 is proteolytically active.

Specifications

• Antigen: Calpain 6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CAPN6
• Gene Alias: calpain 6; calpain-6; Calpain-like protease X-linked; calpamodulin; CalpM; CANPX; Capa6; CAPN6; CAPNX; DJ914P14.1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 83685
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.14 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O88501
• Gene Symbols: CAPN6
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CAPN6 (NP_0551042).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG