Invitrogen™ CA9 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHCA9X10

Product Code. 17767883

Description

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

Specifications

• Accession Number: Q16790
• Assay Sensitivity: 0.2 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CA9
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 0.205 to 50 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 768
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 20 μL|Serum, 20 μL|Supernatant, 100 μL
• Target: Carbonic Anhydrase IX
• Total Assay Time: 4 hr 45 min