C32 (human amelanotic melanoma) Whole cell lysate, Non-denatured; Abnova

C32 (human amelanotic melanoma) whole cell lysate

Brand:  Abnova L002V2.200ug

Product Code. 16009495

Description

• Whole cell lysate (non-denatured)
• Tissue: skin
• Host: Human
• Preparation: homogenization in ice-cold modified RIPA Lysis
• Lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, 1mM PMSF
• Storage buffer: in modified RIPA lysis buffer

Western blotting, immunoprecipitation

Specifications

• For Use With (Application): Immunoprecipitation, Western Blot
• Description: Whole cell lysate (non-denatured)
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
• Quantity: 200 μg
• Host Species: Human
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.

• Tissue: Skin
• Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Concentration: 5 mg/mL