c-Myc, clone: 9E10, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6784-82

Product Code. 15277947

Description

Description: The 9E10 antibody reacts with human c-Myc p67; the antibody was raised against a synthetic peptide corresponding to amino acids 408-439 within the carboxy terminal domain of human c-Myc. This antibody can be used to detect myc-tagged protein. The transcription factor c-Myc is a proto-oncogene that is at the focal point in cell cycle regulation, metabolism, apoptosis, differentiation, cell adhesion, and tumorigenesis. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. C-Myc also plays a pivotal role in apoptosis, most notably its connections to the CD95/Fas death receptor pathway. These different biological responses to c-Myc are most likely the result of different overlapping subsets of c-Myc target genes.Applications Reported: This 9E10 antibody has been reported for use in immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 9E10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 ug/mL. This 9E10 antibody has also been tested by western blot and can be used 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: c-Myc
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01106
• Gene Alias: c Myc, Cellular myelocytomatosis oncogene, Myc protein, Myc proto oncogene protein, Myc2, Niard, Nird, v myc avian myelocytomatosis viral oncogene hom
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: ImmunohistochemistryþImmunoprecipitationþWestern Blot
• Clone: 9.00E+10
• Conjugate: Unconjugated
• Gene: MYC
• Format: Purified
• Gene Symbols: MYC
• Isotype: IgG1
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C.
• Primary or Secondary: Primary
• Gene ID (Entrez): 4609

For Research Use Only.