Invitrogen™ c-MAF Monoclonal Antibody (sym0F1), PE-Cyanine7, eBioscience™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
16527000	Invitrogen™ 25-9855-82	100 μg

Description

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well. Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This sym0F1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins' located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated...

c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.

Specifications

• Antigen: c-MAF
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O75444, P54843
• Gene ID (Entrez): 17132, 4094
• Immunogen: Full-length E. coli-produced c-Maf.
• Product Type: Antibody

• Clone: sym0F1
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2b κ
• Target Species: Human, Mouse
• Gene: MAF
• Gene Alias: 2810401A20Rik; A230108G15Rik; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog; AW047063; AYGRP; basic domain leucine zipper transcription factor; CCA4; cMaf; C-Maf; c-Maf long form; c-maf proto-oncogene; CTRCT21; fl13d12; hMafA; LOW QUALITY PROTEIN: transcription factor Maf; LOW QUALITY PROTEIN: transcription factor MafA; maf; MAF bZIP transcription factor; MAF bZIP transcription factor A; maf protein; Maf2; MAFA; Mafa homolog; MGC71685; pancreatic beta-cell-specific transcriptional activator; Proto-oncogene c-Maf; RGD1562627; RIPE3b1; RIPE3b1 factor; T lymphocyte c-maf long form; transcription factor Maf; Transcription factor Maf-2; transcription factor MafA; transcription factor RIPE3b1; v-maf avian musculoaponeurotic fibrosarcoma oncogene A; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog a (paralog a); v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A; v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian); v-maf musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; wu:fl13d12; Z-cmaf
• Gene Symbols: MAF
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography