Invitrogen™ c-Jun (Total) Multispecies InstantOne™ ELISA Kit

Product Code. 15550807

Brand: Invitrogen™ 858616111

Description

Includes

• User Manual
• InstantOne™ Assay Plate - 96 well (sealed)
• Cell Lysis Buffer (5X)
• Enhancer Solution
• Wash Buffer (10X)
• c-Jun (Total) Capture Antibody Reagent
• c-Jun (Total) Detection Antibody Reagent
• Positive Control Cell Lysate (lyophilized)
• Detection Reagent (TMB Substrate)
• Stop Solution
• Plate Seal

InstantOne ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of total human and mouse c-Jun in cell lysates. Detection of rat c-Jun is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the ′leucine-zipper′ motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes.

Specifications

• Accession Number: P05412,P05627
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Cell Lysates
• Target Species: Human, Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 16476, 3725
• Gene Symbol: AP-1,AP1,c-Jun
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate,20 μL
• Storage Requirements: 2°C to 8°C
• Synonym: JUN, Transcription factor AP-1
• Target: Human, Mouse
• Test Time: 1 hr. 20 min.
• Total Assay Time: 1 hr. 10 min.

For Research Use Only