Invitrogen™ BYSL Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA588285

Product Code. 16556571

Description

Immunogen sequence: HAEVVVDPED ERAIEMFMNK NPPARRTLAD IIMEKLTEKQ TEVETVMSEV SGFPMPQLDP RVLEVYRGVR EVLSKYRSGK LPKAFKIIPA LSNWEQILYV TEPEAWTAAA MYQATRIFAS NLKERMAQRF YNLVLLPRVR DDVAEYKRLN FHLYMALKKA L; Positive Samples: HeLa, Jurkat, A-549, BxPC-3, Mouse testis, Mouse lung, Rat testis, Rat lung.

Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi.

Specifications

• Antigen: BYSL
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BYSL
• Gene Alias: by the ribosomal protein s6 gene, drosophila, homolog-like; Bys; BYSL; bysl {ECO:0000312; Bystin; bystin like; bystin-like; EMBL:AAH79030.1, ECO:0000312; Enp1; RGD:727959}
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 359727, 53414, 705
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.03 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: O54825, Q13895, Q80WL2
• Gene Symbols: BYSL
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human BYSL (NP_0040443).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG