Invitrogen™ BUBR1 Monoclonal Antibody (8G1)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA116577

Product Code. 11518263

Description

Suggested positive control: Hela whole cell extract, antigen standard for BUB1B (transient overexpression lysate).

BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

Specifications

• Antigen: BUBR1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: O60566, Q9Z1S0
• Gene Symbols: BUB1B
• Immunogen: A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 8G1
• Conjugate: Unconjugated
• Gene: BUB1B
• Gene Alias: AU045529; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; BUB1 mitotic checkpoint serine/threonine kinase B; Bub1A; Bub1b; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 beta; budding uninhibited by benzimidazoles 1 homolog beta; budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae); hBub1; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint kinase MAD3L; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; Protein SSK1; SSK1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12236, 171576, 701
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid