Invitrogen™ Bub1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA595995

Product Code. 16598831

Description

Immunogen sequence: MDTPENVLQM LEAHMQSYKG NDPLGEWERY IQWVEENFPE NKEYLITLLE HLMKEFLDKK KYHNDPRFIS YCLKFAEYNS DLHQFFEFLY NHGIGTLSSP LYIAWAGHLE AQGELQHASA VLQRGIQNQA EPREFLQQQY RLFQTRLTET HLPAQARTSE PLHNVQVLNQ MITSKSNPGN NMACISKNQG SELSGVISSA CDKESNMERR VITISKSEYS VHSSLASKVD VEQVVMYCKE KLIRGESEFS FEELRAQKYN QRRKHEQWVN EDRHYMKRKE ANAFEEQLLK QKMDELHKKL; Positive Samples: 293T, HepG2, MCF7, HeLa, SW480, Mouse testis; Cellular Location: Chromosome, Nucleus, centromere, kinetochore.

The BUB1 gene encodes a serine/threonine-protein kinase that is integral to the spindle assembly checkpoint, a crucial mechanism ensuring proper chromosome alignment and segregation during mitosis. BUB1 is involved in detecting and correcting errors in kinetochore-microtubule attachments, which is vital for maintaining genomic stability. It acts by delaying the onset of anaphase until all chromosomes are correctly attached to the spindle apparatus. BUB1 is recruited to kinetochores in early mitosis, where it phosphorylates several substrates to activate other checkpoint proteins, such as BUBR1 and MAD2, thereby preventing premature progression through mitosis. Mutations or aberrations in the expression levels of BUB1 are often associated with aneuploidy and chromosomal instability, contributing to cancer progression. BUB1 is thus considered a potential target for cancer therapeutics, aiming to exploit its role in the mitotic checkpoint to selectively eliminate tumor cells through checkpoint bypass or mitotic arrest.

Specifications

• Antigen: Bub1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BUB1
• Gene Alias: Aamp; Aamp-rs; AL022991; angio associated migratory cell protein; angio-associated migratory cell protein; angio-associated migratory protein; angio-associated, migratory cell protein; AU040907; BUB1; BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1 mitotic checkpoint serine/threonine kinase; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; BUB1L; budding uninhibited by benzimidazoles 1 homolog; budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae); C80208; D2Xrf87; hBUB1; mBUB1; mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12235, 699
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 2.8 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O08901, O43683
• Gene Symbols: BUB1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_0043271).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG