Invitrogen™ Bub1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA578687

Product Code. 15934275

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human K562 whole cell, human HEK293 whole cell, human Hela whole cell, human Jurkat whole cell, human HL-60 whole cell, rat testis tissue, rat heart tissue, rat PC-12 whole cell, mouse testis tissue, mouse thymus tissue, mouse heart tissue, mouse NIH/3T3 whole cell.

The BUB1 gene encodes a serine/threonine-protein kinase that is integral to the spindle assembly checkpoint, a crucial mechanism ensuring proper chromosome alignment and segregation during mitosis. BUB1 is involved in detecting and correcting errors in kinetochore-microtubule attachments, which is vital for maintaining genomic stability. It acts by delaying the onset of anaphase until all chromosomes are correctly attached to the spindle apparatus. BUB1 is recruited to kinetochores in early mitosis, where it phosphorylates several substrates to activate other checkpoint proteins, such as BUBR1 and MAD2, thereby preventing premature progression through mitosis. Mutations or aberrations in the expression levels of BUB1 are often associated with aneuploidy and chromosomal instability, contributing to cancer progression. BUB1 is thus considered a potential target for cancer therapeutics, aiming to exploit its role in the mitotic checkpoint to selectively eliminate tumor cells through checkpoint bypass or mitotic arrest.

Specifications

• Antigen: Bub1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BUB1
• Gene Alias: Aamp; Aamp-rs; AL022991; angio associated migratory cell protein; angio-associated migratory cell protein; angio-associated migratory protein; angio-associated, migratory cell protein; AU040907; BUB1; BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1 mitotic checkpoint serine/threonine kinase; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; BUB1L; budding uninhibited by benzimidazoles 1 homolog; budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae); C80208; D2Xrf87; hBUB1; mBUB1; mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12235, 296137, 699
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene Accession No.: O08901, O43683
• Gene Symbols: BUB1
• Immunogen: E.coli-derived human Bub1 recombinant protein (Position: V731-K1085).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG