Invitrogen™ Brevican Monoclonal Antibody (N294A/6)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA527639

Product Code. 16332154

Description

1 μg/mL of MA5-27639 was sufficient for detection of Brevican in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 140kDa (and smaller due to proteolytic cleavage). This antibody was formerly sold as clone S294A-6.

Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.

Specifications

• Antigen: Brevican
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P55068, Q61361, Q96GW7
• Gene Symbols: Bcan
• Immunogen: Fusion protein amino acids 219-655 of rat Brevican.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Western Blot, Immunocytochemistry
• Clone: N294A/6
• Conjugate: Unconjugated
• Gene: Bcan
• Gene Alias: ALPBRE; Bcan; Behab; Brain-enriched hyaluronan-binding protein; brevican; brevican (brain specific proteoglycan in the aggrecan family); brevican core protein; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB; CSPG7; UNQ2525/PRO6018
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12032, 25393, 63827
• Content And Storage: -20°C
• Form: Liquid