Invitrogen™ BrdU Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA532256

Product Code. 13456777

Description

Recommended positive controls: HeLa cells were treated with 100uM BrdU for 4hrs, U343 cells were treated with 10 μM BrdU for 18hrs. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Specifications

• Antigen: BrdU
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
• Immunogen: BrdU.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Chemical

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

Product Identifier

• ALG9 ANTIBODY

Signal Word

• Warning

Hazard Category

• Skin sensitiser Category 1

Hazard Statement

• H317-May cause an allergic skin reaction.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Mixture, 3(2H)-isothiazolone, 5-chloro-2-methyl-2-methyl-3(2H)-isothiazolone