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BrdU, PE, clone: BU20A, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-5071-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15526996

  • £62.70 / 25 tests


EU Stock 5
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Description

Description

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment. For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol.Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This BU20A antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Staining Buffer Set (cat. 00-5523) or the BrdU Staining Buffer Set (cat. 00-5525) and protocol. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

BrdU
BU20A
PE
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Bromodeoxyuridine
IgG1, kappa
RUO
Primary
Rat
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