BrdU, clone: BU20A, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5071-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionResuspend the cell pellet by pulse vortexing. Then add 1 mL of freshly prepared Foxp3 Fixation/Permeabilization solution (cat. 00-5521) to each sample. pulse vortex again.6. Incubate for 30 to 60 minutes at 2-8°C in the dark.7. Wash once by adding 2 mL 1X Permeabilization Buffer (made from 10X Permeabilization Buffer included in the Foxp3/Transcription Factor Staining Buffer Set) followed by centrifugation. Decant the supernatant.8. Resuspend the cell pellet with 100 µL Flow Cytometry Staining Buffer containing 30 µg of DNase I.9. Incubate for 1 hr at 37°C and then wash.10. Stain cells with anti-BrdU antibody for 30 min to 1 hr and then wash.10. If necessary, stain with secondary antibody for 30 min and then wash.12. Analyze the samples. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
|PBS with 0.09% sodium azide; pH 7.2|
|Flow Cytometry, Immunohistochemistry (Frozen)|
For Research Use Only.