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BrdU Monoclonal Antibody (BU-1), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA3071

Code : Z3

Additional Details : Weight : 0.01000kg

Product Code. 10009169

  • £344.00 / 100µL
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Description

Description

MA3-071 detects 5-Bromo-2-deoxyuridine (BrdU) in many samples. MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications. The MA3-071 immunogen is 5-iodouridine covalently coupled to ovalbumin. This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
Specifications

Specifications

BrdU
Monoclonal
0.05 mg/mL
0.01M HEPES with 0.15M NaCl, 30% glycerol and 0.01% sodium azide
Mouse
Protein A
RUO
Chemical
Antibody
IgG2a
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
BU-1
Unconjugated
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
5-iodouridine covalently coupled to ovalbumin.
100 μL
Primary
-20°C
Liquid
Chemical
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