Invitrogen™ BRCC3 Recombinant Rabbit Monoclonal Antibody (PSH05-10)

Rabbit Recombinant Monoclonal Antibody

Brand:  Invitrogen™ MA556411

Product Code. 30275217

Description

BRCC3 Recombinant Monoclonal Antibody for Western Blot, ICC/IF, IHC (P), Flow

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Specifications

• Antigen: BRCC3
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: B2RYM5, P46736, P46737
• Gene Symbols: BRCC3
• Immunogen: Recombinant protein within human BRCC36 aa 1-316/316.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: PSH05-10
• Conjugate: Unconjugated
• Gene: BRCC3
• Gene Alias: 6.1A protein; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRCA1/BRCA2-containing complex, subunit 3; BRCA1-A complex subunit BRCC36; Brcc3; BRCC36; BRISC complex subunit BRCC36; C6.1A; CXorf53; lys-63-specific deubiquitinase BRCC36; RP11-143H17.2
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 210766, 316794, 79184
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid