Invitrogen™ BRCC3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA565526

Product Code. 15908274

Description

Immunogen sequence: PSHVDVRTQAM YQMMDQGFVG LIFSCFIEDK NTKTGRVLYT CFQSIQAQKS SEYERIEIPI HIVPHVTIGK VCLESAVELP KILCQEEQDA YRRIHSLTHL DSVTKIHNGS VFTKNLCSQM SAVSGPLLQW LEDRLE Highest antigen sequence identity to the following orthologs - mouse 99%, rat 97%.

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Specifications

• Antigen: BRCC3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BRCC3
• Gene Alias: 6.1A protein; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRCA1/BRCA2-containing complex, subunit 3; BRCA1-A complex subunit BRCC36; Brcc3; BRCC36; BRISC complex subunit BRCC36; C6.1A; CXorf53; lys-63-specific deubiquitinase BRCC36; RP11-143H17.2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 79184
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P46736
• Gene Symbols: BRCC3
• Immunogen: Recombinant Human BRCC3. Recombinant protein control fragment (Product #RP-106198).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG