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Invitrogen™ IL-6 Bovine Uncoated ELISA Kit

Uncoated ELISA Kit

Brand:  Invitrogen™ ESS0029

Code : PN

Additional Details : Weight : 0.10000kg

Product Code. 10495335

  • £431.00 / 1 set


In stock, ready to ship: 2
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Description

Description

The Bovine IL-6 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-6. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
Specifications

Specifications

P26892
ELISA Kit
Biotin
280826
Absorbance at 450nm minus absorbance at 550nm
Serum, 100 μL|Supernatant, 100 μL
2°C to 8°C
24 hr.
Bovine
Bovine
<78.1 pg/mL
Colorimetric ELISA (TMB substrate) for 96-well microplates
HRP
78.1-5000 pg/mL
Bovine IL-6 ELISA Reagent Kit
Coating Antibody, Lyophilized Recombinant Standard, Detection Antibody, Streptavidin-HRP, Substrate Solution, Ready to use Stop Solution, 0.16M Sulfuric Acid, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
RUO
No significant cross-reaction with recombinant bovine IL-1ß, IL-2, IL-4, IL-8, IFNg, or TNFa
5 x 96 microplate wells
2 hr. 50 min.
5 x 96 Tests
Supernatant
IL6
Colorimetric Microplate Reader
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