Invitrogen™ IL-2 Bovine ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EBIL2

Product Code. 15444873

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Bovine Interleukin 2 (Bov IL-2) ELISA quantitates Bov IL-2 in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov IL-2. Principle of the method The Bovine IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P05016
• Assay Sensitivity: 0.2 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 280822
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Bovine
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 0.273-200 pg/mL
• Calibration Range: 0.2 ng/ml to 200 ng/ml
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: Detects bovine IL-2 in this Sandwich ELISA. It shows less than 0.2% cross-reactivity with the recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant cotton rat IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant feline IL-2.
• Target Species: Bovine
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL2
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: BOS_16374, IL-2, TCGF
• Test Time: 1 hr. 20 min.